Molecular methods in ecology and nature conservation WF-OB-MOL
Presents methods of collecting and storing samples for molecular studies. It presents the most important molecular markers and their application in ecological and nature protection studies
Lecture:
What does the molecular ecology aim with? Basics concepts of genetics and molecular ecology
Sources of DNA, methods of sampling, DNA isolation
Molecular techniques and molecular markers in ecology and nature conservation
Genetic relatedness between individuals, population genetic structure and gene flow
Molecular methods –relatedness between individuals, genetic structure of populations, gene flow, reproductive systems, sex ratio variation, migrations of individuals, population size, genetic variation, inbreeding, ect.
Laboratory:
Safety in the laboratory, acquaintance with equipment and standards of work in the lab
DNA isolation from blood and other tissues (various methods), determination of quality and quantity of isolated DNA (spectrophotometer operation)
Bird molecular sexing techniques - PCR preparation, principle of operation of thermocycler
DNA electrophoresis and visualization of electrophoresis products
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Term 2021/22_L:
Lecture: Laboratory: |
Term 2022/23_L:
Lecture: Laboratory: |
Term 2023/24_L:
Lecture: Laboratory: |
Term 2024/25_L:
Presents methods of collecting and storing samples for molecular studies. It presents the most important molecular markers and their application in ecological and nature protection studies Lecture: Laboratory: |
(in Polish) Dyscyplina naukowa, do której odnoszą się efekty uczenia się
(in Polish) E-Learning
(in Polish) Grupa przedmiotów ogólnouczenianych
(in Polish) Opis nakładu pracy studenta w ECTS
Subject level
Learning outcome code/codes
Type of subject
Preliminary Requirements
Course coordinators
Learning outcomes
a) Learning outcomes
1. Knowledge:
EK1 - student knows methods of molecular ecology and their usage in ecology and conservation of nature, describes and identifies types of molecular techniques and markers, defines concepts in the field of genetics and molecular ecology
EK2 – student recognizes the basic terms in the field of molecular methods used in ecology and nature conservation in a foreign language (English)
2. Skills:
EK3 – student is able to perform simple tasks in the field of molecular research and safely uses the devices in the laboratory
EK4 – student uses specialized terminology in the field of molecular ecology and conservation
3. Competences:
EK5 – student expands and improves professional skills in the laboratory research methods
EK6 - student cares about the reliability and credibility of their work
Assessment criteria
Laboratory
Average score from 3 exercise entries (5 multiple choice/open questions).
3 correct answers - 3
4 correct answers - 4
5 correct answers - 5
Students will be assessed for compliance with occupational health and safety rules during classes. 1 absence from classes is allowed (excused or unexcused).
Lecture
Assessment of the presentation of the article (mostly English) related to the topic or presentation related to the topic. The evaluation will assess the ability present a multimedia presentation (form, content and way of presentation), the ability to find suitable material for presentation -50% of final evaluation
Final test - 50% of evaluation
Final test assesing knowledge - mainly single choice questions, also questions about presentations
A total of test points above 55% passes the subject.
from 55.1% - 3
from 65.1% - 3+
from 70.1% - 4
from 80.1% - 4+
from 85.1% - 5
Prerequisite to take the final test: to complete the laboratory, to make a presentation, and to attend at least half of the lectures.
Attendance at the lecture will be checked.
Further detailed assessment criteria will be given to participants during the course.
Bibliography
Selected chapters from:
1. J.R. Freeland 2008. Ekologia molekularna. PWN, Warszawa
2. M. Pilot, R.Rutkowski, A. Malewska, T. Malewski 2005. Zastosowanie metod molekularnych w badaniach ekologicznych. Muzeum i Instytut Zoologii PAN, Warszawa
Additional materials:
Bukacińska M., Bukaciński D., Epplen J.T., Sauer K.P., Lubjuhn T. 1998. Low frequency of extra-pair paternity in Common Gulls (Larus canus) as revealed by DNA fingerprinting. J. Ornithol. 139: 413-420.
Bukaciński D., Bukacińska M., Lubjuhn T. 2000. Adoption of chicks and the level of relatedness in common gull, Larus canus colonies: DNA fingerprinting analyses. Anim. Behav. 59: 289-299.
Bukaciński D., Bukacińska M., Buczyński A. 2020. Offspring Sex Ratio in the First and Replacement Clutches of the Mew Gulls (Larus canus): Breeding Tactics in the Riverine Population of a Sexually Size-dimorphic Bird. Waterbirds 43 (2): 174-185
P.C. Winter, G.I. Hickey, H.L. Fletcher 2006. Krótkie wykłady. Genetyka. PWN, Warszawa
P.C. Turner, A.G. McLennan, A.D. Bates, M.R.H. White 2007. Krótkie wykłady. Biologia molekularna. PWN, Warszawa
J.C. Avise 2004. Markery molekularne, historia naturalna i ewolucja. Wydawnictwo Uniwersytetu Warszawskiego, Warszawa
D.J. Futuyma 2005. Ewolucja. Wydawnictwo Uniwersytetu Warszawskiego, Warszawa
Scientific papers in the field of molecular ecology (using of molecular methods in ecology and nature conservation) – given by lecturer and presented by students (mostly in English)
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Term 2021/22_L:
1. J.R. Freeland 2008. Ekologia molekularna. PWN, Warszawa Additional materials: |
Term 2022/23_L:
1. J.R. Freeland 2008. Ekologia molekularna. PWN, Warszawa Additional materials: |
Term 2023/24_L:
1. J.R. Freeland 2008. Ekologia molekularna. PWN, Warszawa Additional materials: |
Term 2024/25_L:
Selected chapters from: Additional materials: Scientific papers in the field of molecular ecology (using of molecular methods in ecology and nature conservation) – given by lecturer and presented by students (mostly in English) |
Notes
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Term 2021/22_L:
basic knowledge of zoology, biochemistry, ecology, genetics, passive English |
Additional information
Information on level of this course, year of study and semester when the course unit is delivered, types and amount of class hours - can be found in course structure diagrams of apropriate study programmes. This course is related to the following study programmes:
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: