Molecular biology WB-BI-35-25
The course on the subject of "Molecular Biology" includes lectures and exercises. The lectures cover basic issues related to molecular biology. The types of genetic information carriers are presented, taking into account the structure of DNA and RNA in the mitochondrial, chloroplast, and nuclear genomes. The processes related to gene expression (transcription and translation) are discussed, paying particular attention to the factors involved in the regulation of these processes. Moving and palindromic sequences, the origin of introns, pseudogenes, polyploidization, duplication, and the formation of new genes are discussed. Repair mechanisms including modern CRISPR / Cas 9 methods and modern methods used in the genetic engineering of organisms - creating genetic constructs, cloning, artificial chromosomes, and genome editing. Traditional and modern methods of genome research are introduced. The basics and assumptions of diagnostic methods in the sciences of population and forensic genetics are presented, based on the analysis of polymorphic molecular markers, including RAPD, RFLP, AFLP, and SSR.
Term 2021/22_Z:
The course on the subject of "Molecular Biology" includes lectures and exercises. The lectures cover basic issues related to molecular biology. The types of genetic information carriers are presented, taking into account the structure of DNA and RNA in the mitochondrial, chloroplast, and nuclear genomes. The processes related to gene expression (transcription and translation) are discussed, paying particular attention to the factors involved in the regulation of these processes. Moving and palindromic sequences, the origin of introns, pseudogenes, polyploidization, duplication, and the formation of new genes are discussed. Repair mechanisms including modern CRISPR / Cas 9 methods and modern methods used in the genetic engineering of organisms - creating genetic constructs, cloning, artificial chromosomes, and genome editing. Traditional and modern methods of genome research are introduced. The basics and assumptions of diagnostic methods in the sciences of population and forensic genetics are presented, based on the analysis of polymorphic molecular markers, including RAPD, RFLP, AFLP, and SSR. |
Term 2022/23_Z:
None |
Term 2023/24_Z:
None |
Term 2024/25_Z:
None |
(in Polish) Dyscyplina naukowa, do której odnoszą się efekty uczenia się
(in Polish) E-Learning
(in Polish) Grupa przedmiotów ogólnouczenianych
(in Polish) Opis nakładu pracy studenta w ECTS
Subject level
Learning outcome code/codes
Type of subject
Preliminary Requirements
Course coordinators
Learning outcomes
Subject effects in terms of knowledge:
Subject effects in terms of knowledge:
Subject effect 1: The graduate knows the rules of building the genetic code at an advanced level, as well as the processes and phenomena of the development and genetic variation of organisms, has a good understanding of the basic phenomena and processes underlying molecular biology
Subject effect 2: has advanced knowledge of the most important problems in various branches of biology, including genetics, cell biology, and biochemistry, necessary for understanding basic natural phenomena and processes, and knows the connections between the above-mentioned sciences and other natural disciplines
Subject effect 3: has advanced knowledge of basic concepts related to DNA, RNA, amino acids, as well as terminology used in molecular biology, and has knowledge of the development of this science and research method used in it today
Subject effect 4: has knowledge of basic techniques and research tools useful for understanding the structure and functions of nucleic acids and proteins, as well as has knowledge of computer science and statistics at a level that allows their use in the analysis of natural phenomena
Subject effect 5: understands the relationship between the achievements of molecular biology and the possibility of its use in socio-economic life, taking into account the sustainable use of biological diversity
Subject effect 6: knows and understands the fundamental dilemmas of modern civilization, including the basic ethical principles of professional work using molecular biology tools, has knowledge of the basic concepts and principles of copyright law regarding selected aspects of molecular biology and related sciences
Objective effects in terms of skills:
Objective effect 7: uses basic techniques of molecular analysis of DNA, RNA and proteins appropriate to the investigated pro-or eukaryotic genome and tools enabling the analysis of polymorphism at the molecular level
Subject effect 8: understands biology literature in Polish; reads and understands simple scientific texts in the field of molecular biology in English
Objective effect 9: uses available information sources, including electronic sources such as NCBI and PubMed
Subject effect 10: performs assigned simple research tasks under the supervision of a research tutor
Subject effect 11: Performs observations and performs simple analyzes in the laboratory used in molecular biology, i.e. PCR
Subject effect 12: learns independently in a targeted manner in the molecular biology of pro-and eukaryotic organisms
Subject effects in the field of social competencies:
Subject Effect 13: Graduate understands the need for lifelong learning in the field of molecular biology
Subject effect 14: can interact and work in a group, assuming different roles in it
Objective effect 15: correctly identifies and resolves dilemmas related to the profession of molecular biology
Objective effect 16: is responsible for the safety of his own and others' work; knows how to act in emergency situations
Subject learning outcomes assigned to lectures (1-6).
Subject learning outcomes assigned to exercises (7-16).
Assessment criteria
Lecture
The following grading scale applies: 94 - 100% very good (5.0), 88 - 93% plus good (4.5), 80 - 87% good (4.0), 70 - 79% plus satisfactory (3.5), 60 - 69% satisfactory (3.0), less than 59.9% insufficient (2.0). The condition for completing the course is attendance at the classes and active participation of the student in the exercises.
Exercises:
Partial tests of known methods of molecular biology research. The student is required to complete the exercise cards during the laboratories, and also have the ability to search and select appropriate sources (scientific articles and electronic sources, also in English). The student should complete the assigned homework.
Final grade based on partial grades obtained during the semester from tests and activity in classes. Classes are credited if the student: (i) actively participated in at least 85% of the classes; (ii) work in the classroom in a way that allows to positively assess the knowledge, skills, and social competencies obtained in the course of the classes (described in the syllabus as subject learning outcomes 7-16).
The range of grades from the colloquium:
94 - 100% - 5
93 - 88% - 4,5
87 - 80% - 4
79 - 70% - 3,5
69 - 60% - 3
mniej niż 59,9% - 2
For the grade 2 (not passed): The graduate does not know the rules of the structure of the genetic code at an advanced level, as well as the processes and phenomena of the development and genetic variation of organisms, does not understand the basic phenomena and processes underlying molecular biology; does not have advanced knowledge of the most important problems in various branches of biology, including genetics, cell biology and biochemistry, necessary for understanding basic natural phenomena and processes, and does not know the connections between the above-mentioned sciences and other natural disciplines; does not have advanced knowledge in the field of basic concepts related to DNA, RNA, amino acids, or terminology used in molecular biology, and has no knowledge of the development of this science and research methods used in it today; he has no knowledge of basic techniques and research tools useful for understanding the structure and functions of nucleic acids and proteins, and no knowledge of computer science and statistics at a level that allows their use in the analysis of natural phenomena; does not understand at all the relationship between the achievements of molecular biology and the possibility of its use in socio-economic life, taking into account the sustainable use of biological diversity; does not know at all and does not understand the fundamental dilemmas of modern civilization, including the basic ethical principles of professional work using molecular biology tools, has no knowledge of the basic concepts and principles of copyright concerning selected aspects of molecular biology and related sciences.
To grade 3 (passed): At the basic level, the graduate knows the principles of the structure of the genetic code at an advanced level, as well as the processes and phenomena of the development and genetic variation of organisms, at the basic level understands the basic phenomena and processes underlying molecular biology; has intermediate knowledge of the most important problems in various areas of biology, including genetics, cell biology and biochemistry, necessary for understanding basic natural phenomena and processes, and knows the connections between the above-mentioned sciences and other natural disciplines; has intermediate knowledge of the basic concepts related to DNA, RNA, amino acids, as well as terminology used in molecular biology, and has a basic knowledge of the development of this science and research methods used today; has basic knowledge of basic research techniques and tools useful for understanding the structure and functions of nucleic acids and proteins, as well as basic knowledge in the field of computer science and statistics at a level that allows their use in the analysis of natural phenomena; at the basic level, understands the relationship between the achievements of molecular biology and the possibility of its use in socio-economic life, taking into account the sustainable use of biological diversity; knows and understands the fundamental dilemmas of modern civilization, including the basic ethical principles of professional work using molecular biology tools, has average knowledge of the basic concepts and principles of copyright law regarding selected aspects of molecular biology and related sciences.
To grade 4 (well): The graduate has a good knowledge of the advanced rules of the structure of the genetic code, as well as the processes and phenomena of the development and genetic variation of organisms, has a good understanding of the basic phenomena and processes underlying molecular biology; has a thoroughly advanced knowledge of the most important problems in various areas of biology, including genetics, cell biology and biochemistry, necessary for understanding basic natural phenomena and processes, and knows the connections between the above-mentioned sciences and other natural disciplines; has advanced knowledge of basic concepts related to DNA, RNA, amino acids, as well as terminology used in molecular biology, and a good knowledge of the development of this science and research methods used today; has good knowledge of basic techniques and research tools useful for understanding the structure and functions of nucleic acids and proteins, as well as good knowledge of computer science and statistics at a level that allows their use in the analysis of natural phenomena; has a good understanding of the relationship between the achievements of molecular biology and the possibility of its use in socio-economic life, taking into account the sustainable use of biological diversity;
To grade 5 (very well): The graduate knows very well the rules of building the genetic code, as well as the processes and phenomena of development and genetic variation of organisms, very well understand the basic phenomena and processes underlying molecular biology; has a very advanced knowledge of the most important problems in various areas of biology, including genetics, cell biology and biochemistry, necessary for understanding basic natural phenomena and processes, and knows the connections between the above-mentioned sciences and other natural disciplines; has a very advanced knowledge of the basic concepts related to DNA, RNA, amino acids, as well as terminology used in molecular biology, and a very good knowledge of the development of this science and research methods used in it today; has a very good knowledge of basic techniques and research tools useful for understanding the structure and functions of nucleic acids and proteins, as well as a very good knowledge of computer science and statistics at a level that allows their use in the analysis of natural phenomena; understands very well the relationship between the achievements of molecular biology and the possibility of its use in socio-economic life, taking into account the sustainable use of biological diversity; knows very well and understands the fundamental dilemmas of modern civilization, including the basic ethical principles of professional work using molecular biology tools, has a very good knowledge of the basic concepts and principles of copyright concerning selected aspects of molecular biology and related sciences.
Bibliography
Compulsory literature:
Molecular biology. Short lectures. P.C. Turner, A.G. McLennan, A.D. Bates, M.R.H. White. PWN SA, Warsaw, 2012
Molecular markers, natural history, and evolution. J.C. Avise. WUW, Warsaw, 2008
Molecular biology in medicine, edited by J. Bal. PWN SA, Warsaw, 2013
Fundamentals of population genetics. D.L. Hartl, A.G. Clark. WUW, Warsaw, 2009
Genetics. Short lectures. P.C. Winter, G.I. Hickey, H.L Fletcher. PWN Warsaw, 2004
Supplementary literature:
DNA Analysis, Theory and Practice. Collective work edited by Ryszard Słomski. Publishing House of the University of Life Sciences in Poznań 2008.
Introduction to Protein Structure. C. Branden, J. Tooze, Garland Publ.
Source materials from PubMed, NCBI, and scientific publications provided by the teacher during the classes.
Term 2021/22_Z:
Compulsory literature: |
Term 2022/23_Z:
None |
Term 2023/24_Z:
None |
Term 2024/25_Z:
None |
Notes
Term 2021/22_Z:
Basic knowledge of genetics from high school. |
Additional information
Information on level of this course, year of study and semester when the course unit is delivered, types and amount of class hours - can be found in course structure diagrams of apropriate study programmes. This course is related to the following study programmes:
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: