Microbiology WB-BI-24-13lab
Laboratory sessions:
1. The rules of practice and principles of work safety in the laboratory. Basic glass and equipment for microbiological lab. Microbiological methods of sterilization.Media and methods of microorganisms cultivation.Microbiological observations.
2. Isolation of microorganisms from different sourses (water, soil). Methods of inoculation on solid medium.
3. Microscopic observation of different forms of microorgannisms. Isolation of pure culture.
4. Morphology of bacteria. Staining methods.
5. Morphology of fungi cells. Observation of yeast and mold cells
6. Detection of redox and proteolytic activity of bacteria. Bacterial activity and lactic acid fermentation
7. Rapid biochemical and serological methods for pathogenic bacteria assessment. Demonstration of Koch's postulates.
8. Detection of bacteria in selected food products.
9. Effects of temperature and selected chemicals on the growth of microorganisms.
10. Interactios between microorganisms. Types of indirect impacts.
11. Determination of microbial sensivity to antibiotics using disks diffusion methods and agar well diffusion plate methods.
12. Sewage and their purification. Microbiological analysis of the water using a membrane filtration methods. Microscopic analysis of activated sludge from wastewater.
13. Methods for quantification of bacteria and fungi. The microbiological analysis of air by Koch sedimantetion method.
14. Final exam
15. Corrective test
Assesment methods: Final exam consisting of combination of multi-choice and short written answer
(in Polish) Dyscyplina naukowa, do której odnoszą się efekty uczenia się
(in Polish) E-Learning
Term 2020/21_L: (in Polish) E-Learning | Term 2019/20_L: (in Polish) E-Learning z podziałem na grupy |
(in Polish) Grupa przedmiotów ogólnouczenianych
Subject level
Learning outcome code/codes
Type of subject
Preliminary Requirements
Course coordinators
Learning outcomes
Knowledge:
Lo 1 - student be able to understand basic microbiological phenomena and process
Lo 2 - student has knowledge of basic conceptual categories and microbiological terminology and is familiar with the development of microbiology and the research methods used in it
Lo 3 - student has knowledge of the basic techniques and research tools used in microbiology
Lo 4 - student knows the basic principles of health and safety in microbiological laboratory
Skills:
Lo 5 - student uses the basic techniques and research tools used in the mucrobiological laboratory
Lo 6 - student performs simple experiments with microorganisms in the laboratory under the supervision of a scientific supervisor
Competencies:
Lo 7 - student is able to interact and work in a group, taking on different roles
Subject effects for lecture (EK 1-2), subject effects for exercises (EK 3-7)
Student activity student workload in hours
participation in lecture 30
participation in laboratory 45
preparation for laboratories 5
time to write the report 15
prepare for the test 15
prepare for the exam 20
TOTAL HOURS 130/30(25) hrs. ≈4
Number ECTS: 4
Assessment criteria
Knoledge (Learning outcomes 3-4)
Grade 2 (fail): student has no knowledge of the basic techniques and research tools used in the microbiology laboratory, knows to a very limited extent the basic principles of occupational health and safety in a microbiological laboratory
Grade 3 (sufficient): student has limited knowledge of basic techniques and research tools used in the microbiological laboratory, knows the basic principles of occupational health and safety in the microbiological laboratory to a limited extent
Grade 4 (good): student has good knowledge of basic techniques and research tools used in the microbiological laboratory, knows the basic principles of occupational health and safety in the microbiological laboratory
Grade 5 (excellent): the student has a very good knowledge of the basic techniques and research tools used in the microbiological laboratory, knows very well the basic principles of occupational health and safety in a microbiological laboratory
Skills ( Learning outcomes 5-6)
Grade 2 (fail): the student uses the basic techniques and research tools used in the microbiological laboratory to a much limited extent, performs simple experiments with microorganisms under the supervision of a scientific supervisor with great difficulty
Grade 3 (sufficient): the student uses the basic techniques and research tools used in the microbiological laboratory to a limited extent, with difficulty performs simple experiments with microorganisms in the laboratory under the supervision of a scientific supervisor
Grade 4 (good): student is able to properly use the basic techniques and research tools used in the microbiological laboratory, knows how to perform simple experiments with microorganisms in the laboratory under the guidance of a scientific supervisor
Grade 5 (excellent): student is able to very well use the basic techniques and research tools used in the microbiological laboratory, he can very well perform simple experiments with microorganisms in the laboratory under the guidance of a scientific supervisor
Competencies (Learning outcomes 7)
Grade 2 (fail): student is unable to interact and work in a group, taking on different roles
Grade 3 (sufficient): student is able to interact to a limited extent and work in a group, taking on different roles in it
Grade 4 (good): student is able to interact well and work in a group, taking on different roles
Grade 5 (excellent): student is able to interact very well and work in a group, taking on different roles in it
The final grade of the laboratory include: evaluation of activities and evaluation of the written test.
At the final evaluation will be taken into account:
1) prepare a report after every experiment (1 - 4 points for each report). When commissioning a report late loss of some points
2) evaluation of the test (written test) 0-30 points.; 16-18 points - 3.0; 19-21pkt. - 3.5; 22-24 points. - 4.0; 25-27 points. - 4.5; 28-30 points. - 5.0
Acceptable two excused absences.
Bibliography
Obligatory literature:
Różalski A. -Ćwiczenia z mikrobiologii ogólnej cz. 1- teoretyczna - Wydawnictwo Uniwersytetu Łódzkiego, Łódź 2007 r.
Supplementary literature:
Nicklin J., Graeme-Cook K., Killington R. - Mikrobiologia - krótkie wykłady, PWN, Warszawa 2019
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: